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Protocol Overview: 10-Day Alternating Cycle Total Duration: 10 days (5 doses of each peptide) Schedule: Alternate peptides every other day (e.g., Day 1 MGF, Day 2 IGF-1 LR3, Day 3 MGF, etc.) Mechanism of Action MGF (Mechano Growth Factor): MGF is a splice variant of IGF-1 that is upregulated in muscle following mechanical overload or damage

Once reconstituted, store refrigerated and use within appropriate timeframes

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Cell proliferation and clonogenic assays The TACS XTT cell proliferation assay kit (Trevigen) was used for cell proliferation assays as described previously